产品描述

This Human BIRC3 ELISA Kit was designed for the quantitative measurement of Human BIRC3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 37.5 pg/mL-2400 pg/mL and the sensitivity is 9.4 pg/mL.

别名

AIP 1 ELISA Kit; AIP1 ELISA Kit; API 2 ELISA Kit; API2 ELISA Kit; Apoptosis inhibitor 2 ELISA Kit; Baculoviral IAP repeat containing 3 ELISA Kit; Baculoviral IAP repeat containing protein 3 ELISA Kit; Baculoviral IAP repeat-containing protein 3 ELISA Kit; BIRC 3 ELISA Kit; BIRC3 ELISA Kit; BIRC3_HUMAN ELISA Kit; C IAP2 ELISA Kit; C-IAP2 ELISA Kit; Cellular inhibitor of apoptosis 2 ELISA Kit; cellular inhibitor of apoptosis protein 2 ELISA Kit; CIAP 2 ELISA Kit; CIAP2 ELISA Kit; HAIP 1 ELISA Kit; HAIP1 ELISA Kit; HIAP 1 ELISA Kit; HIAP-1 ELISA Kit; HIAP1 ELISA Kit; IAP homolog C ELISA Kit; IAP-1 ELISA Kit; Inhibitor of apoptosis protein 1 ELISA Kit; MALT 2 ELISA Kit; MALT2 ELISA Kit; Mammalian IAP homolog C ELISA Kit; MIHC ELISA Kit; RING finger protein 49 ELISA Kit; RNF49 ELISA Kit; TNFR2 TRAF signaling complex protein 1 ELISA Kit; TNFR2 TRAF signalling complex protein ELISA Kit; TNFR2-TRAF-signaling complex protein 1 ELISA Kit

缩写

BIRC3

Uniprot No

Q13489

灵敏度

9.4 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human BIRC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		91
		Range %		86-94
1:2		Average %		92
		Range %		87-95
1:4		Average %		94
		Range %		87-98
1:8		Average %		103
		Range %		96-107

回收率

The recovery of human BIRC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		86-94
EDTA plasma (n=4)		103		98-107

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 2400 2.154 2.274 2.214 2.112 1200 1.268 1.318 1.293 1.191 600 0.741 0.798 0.770 0.668 300 0.443 0.460 0.452 0.350 150 0.292 0.285 0.289 0.187 75 0.167 0.174 0.171 0.069 37.5 0.132 0.139 0.136 0.034 0 0.101 0.102 0.102

货期

3-5 working days