产品描述
This Bovine Casp7 ELISA Kit was designed for the quantitative measurement of Bovine Casp7 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.
缩写
Casp7
Uniprot No
F1MD58
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine Casp-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 85-101 | |
| 1:2 | Average % | 95 | |
| Range % | 88-102 | |
| 1:4 | Average % | 89 | |
| Range % | 83-100 | |
| 1:8 | Average % | 93 | |
| Range % | 89-106 | |
回收率
| The recovery of bovine Casp-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 90-112 | |
| EDTA plasma (n=4) | 93 | 84-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.546 | 2.457 | 2.502 | 2.369 | | | 20 | 1.883 | 1.905 | 1.894 | 1.761 | | | 10 | 1.136 | 1.124 | 1.130 | 0.997 | | | 5 | 0.726 | 0.711 | 0.719 | 0.586 | | | 2.5 | 0.407 | 0.397 | 0.402 | 0.269 | | | 1.25 | 0.305 | 0.313 | 0.309 | 0.176 | | | 0.625 | 0.196 | 0.204 | 0.200 | 0.067 | | | 0 | 0.130 | 0.136 | 0.133 | | |
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货期
3-5 working days