产品描述
This Mouse CTLA4 ELISA Kit was designed for the quantitative measurement of Mouse CTLA4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
Ctla4 ELISA Kit; Cd152 ELISA Kit; Cytotoxic T-lymphocyte protein 4 ELISA Kit; Cytotoxic T-lymphocyte-associated antigen 4 ELISA Kit; CTLA-4 ELISA Kit; CD antigen CD152 ELISA Kit
缩写
CTLA4
Uniprot No
P09793
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTLA-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 97 | | |
| Range % | 95-99 | | |
| 1:2 | Average % | 102 | | |
| Range % | 97-106 | | |
| 1:4 | Average % | 86 | | |
| Range % | 83-89 | | |
| 1:8 | Average % | 94 | | |
| Range % | 90-98 | | |
回收率
| The recovery of mouse CTLA-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 102 | 96-108 | | |
| EDTA plasma (n=4) | 87 | 83-90 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | | 20 | 2.934 | 2.868 | 2.901 | 2.725 | | | | 10 | 2.312 | 2.301 | 2.307 | 2.131 | | | | 5 | 1.537 | 1.602 | 1.570 | 1.394 | | | | 2.5 | 0.951 | 0.987 | 0.969 | 0.793 | | | | 1.25 | 0.637 | 0.629 | 0.633 | 0.457 | | | | 0.625 | 0.386 | 0.374 | 0.380 | 0.204 | | | | 0.312 | 0.298 | 0.300 | 0.299 | 0.123 | | | | 0 | 0.175 | 0.177 | 0.176 | | | |
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货期
3-5 working days