产品描述

This Human CTSA ELISA Kit was designed for the quantitative measurement of Human CTSA protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

beta galactosidase 2 ELISA Kit; BETA GALACTOSIDASE PROTECTIVE PROTEIN ELISA Kit; beta-galactosidase 2 ELISA Kit; beta-galactosidase protective protein ELISA Kit; betagalactosidase 2 ELISA Kit; Carboxypeptidase C ELISA Kit; Carboxypeptidase L ELISA Kit; carboxypeptidase Y-like kininase ELISA Kit; Cathepsin A ELISA Kit; Ctsa ELISA Kit; deamidase ELISA Kit; EC 3.4.16.5 ELISA Kit; Glactosialidosis ELISA Kit; GLB2 ELISA Kit; Goldberg Syndrome ELISA Kit; GSL ELISA Kit; lysosomal carboxypeptidase A ELISA Kit; Lysosomal protective protein 20 kDa chain ELISA Kit; Lysosomal protective protein ELISA Kit; Lysosomal protective protein deficiency ELISA Kit; NEURAMINIDASE BETA GALACTOSIDASE EXPRESSION; NGBE ELISA Kit; Neuraminidase deficiency with beta-galactosidase deficiency ELISA Kit; NGBE ELISA Kit; OTTHUMP00000031778 ELISA Kit; OTTHUMP00000031781 ELISA Kit; PPCA ELISA Kit; PPCA deficiency ELISA Kit; PPGB ELISA Kit; PPGB_HUMAN ELISA Kit; Protective protein cathepsin A ELISA Kit; Protective protein for beta galactosidase ELISA Kit; Protective protein for beta-galactosidase ELISA Kit; Protective protein/cathepsin A deficiency ELISA Kit; urinary kininase ELISA Kit

缩写

CTSA

Uniprot No

P10619

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CTSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		88-96
1:2		Average %		89
		Range %		85-93
1:4		Average %		106
		Range %		102-109
1:8		Average %		94
		Range %		87-98

回收率

The recovery of human CTSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		85-96
EDTA plasma (n=4)		96		92-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.745 2.849 2.797 2.693 500 2.257 2.412 2.335 2.231 250 1.616 1.717 1.667 1.563 125 0.943 0.965 0.954 0.850 62.5 0.547 0.524 0.536 0.432 31.2 0.354 0.363 0.359 0.255 15.6 0.245 0.254 0.250 0.146 0 0.103 0.105 0.104

货期

3-5 working days