产品描述
CD81(Cluster of Differentiation 81),又称为TAPA-1 (Target of the antiproliferative antibody 1),是一种细胞表面蛋白,广泛存在于各种细胞类型中,包括B和T淋巴细胞。CD81在免疫应答中起着重要作用,它参与B和T细胞的激活、增殖、分化等过程,同时还参与多种病毒感染。人体内CD81的含量与免疫系统的功能紧密相关。
华美生物所提供的Human CD81 antigen(CD81) ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆、细胞裂解液样本中的CD81,其灵敏度为0.039 ng/mL,检测范围为0.156 ng/mL-10 ng/mL。
别名
26 kDa cell surface protein TAPA 1 ELISA Kit; 26 kDa cell surface protein TAPA-1 ELISA Kit; 26 kDa cell surface protein TAPA1 ELISA Kit; CD 81 ELISA Kit; CD81 ELISA Kit; CD81 antigen (target of antiproliferative ELISA Kit 1) ELISA Kit; CD81 antigen ELISA Kit; CD81 molecule ELISA Kit; CD81_HUMAN ELISA Kit; CVID6 ELISA Kit; S5.7 ELISA Kit; TAPA 1 ELISA Kit; TAPA1 ELISA Kit; Target of the antiproliferative ELISA Kit 1 ELISA Kit; Tetraspanin 28 ELISA Kit; Tetraspanin-28 ELISA Kit; Tetraspanin28 ELISA Kit; Tspan 28 ELISA Kit; Tspan-28 ELISA Kit; Tspan28 ELISA Kit
缩写
CD81
Uniprot No
P60033
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD81 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-101 |
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| 1:2 |
Average % |
97 |
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| Range % |
92-105 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-100 |
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| 1:5 |
Average % |
95 |
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| Range % |
90-100 |
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回收率
| The recovery of human CD81 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
95-100 |
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| EDTA plasma (n=4) |
98 |
94-102 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.185 |
2.064 |
2.125 |
1.977 |
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| 5 |
1.407 |
1.381 |
1.394 |
1.246 |
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| 2.5 |
0.807 |
0.790 |
0.799 |
0.651 |
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| 1.25 |
0.451 |
0.434 |
0.443 |
0.295 |
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| 0.625 |
0.326 |
0.352 |
0.339 |
0.191 |
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| 0.312 |
0.269 |
0.273 |
0.271 |
0.123 |
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| 0.156 |
0.197 |
0.204 |
0.201 |
0.053 |
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| 0 |
0.149 |
0.146 |
0.148 |
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货期
3-5 working days