产品描述
The rat α-SMA ELISA Kit is engineered for accurate measurement of rat α-SMA levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the α-SMA content in the sample. The color intensity is positively correlated with α-SMA content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.
α-SMA, also called ACTA2, is the actin isoform that predominates within vascular smooth muscle cells. The expression of α-SMA defines the phenotypic transition to myofibroblasts and is a major contributor to increased contractile force generation by these cells. α-SMA contributes to cell-generated mechanical tension and is normally restricted to cells of vascular smooth muscle. It is used as a marker for a subset of activated fibrogenic cells, myofibroblasts, which are important effector cells of tissue fibrogenesis. α-SMA is important dor fibrogenesis. Additionally, α-SMA can enhance the contractile activity of myofibroblasts.
别名
Acta2 ELISA kit; Actsa ELISA kit; ActvsActin ELISA kit; aortic smooth muscle ELISA kit; Alpha-actin-2) [Cleaved into: Actin ELISA kit; aortic smooth muscle ELISA kit; intermediate form] ELISA kit
缩写
ACTA2
Uniprot No
P62738
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat α-SMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 97 | |
| Range % | 92-101 | |
| 1:2 | Average % | 90 | |
| Range % | 85-94 | |
| 1:4 | Average % | 87 | |
| Range % | 82-91 | |
| 1:8 | Average % | 102 | |
| Range % | 97-109 | |
回收率
| The recovery of rat α-SMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 90-100 | |
| EDTA plasma (n=4) | 93 | 89-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 50 | 2.595 | 2.451 | 2.523 | 2.431 | | | 25 | 1.689 | 1.601 | 1.645 | 1.553 | | | 12.5 | 0.972 | 0.893 | 0.933 | 0.841 | | | 6.25 | 0.493 | 0.512 | 0.503 | 0.411 | | | 3.13 | 0.330 | 0.306 | 0.318 | 0.226 | | | 1.56 | 0.206 | 0.210 | 0.208 | 0.116 | | | 0.78 | 0.126 | 0.121 | 0.124 | 0.032 | | | 0 | 0.094 | 0.089 | 0.092 | | |
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货期
3-5 working days
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