产品描述
This Rabbit Col ELISA Kit was designed for the quantitative measurement of Rabbit Col protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL.
别名
Collagen Type 4(COL 4),_x000D_
Collagen Type IV(COL IV)
缩写
Col
灵敏度
3.12 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
84-91 |
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| 1:2 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:4 |
Average % |
98 |
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| Range % |
95-104 |
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| 1:8 |
Average % |
86 |
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| Range % |
80-94 |
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回收率
| The recovery of rabbit COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
85 |
81-92 |
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| EDTA plasma (n=4) |
93 |
87-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 800 |
2.734 |
2.534 |
2.634 |
2.541 |
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| 400 |
2.055 |
2.135 |
2.095 |
2.002 |
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| 200 |
1.348 |
1.324 |
1.336 |
1.243 |
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| 100 |
0.677 |
0.669 |
0.673 |
0.580 |
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| 50 |
0.412 |
0.421 |
0.417 |
0.324 |
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| 25 |
0.278 |
0.274 |
0.276 |
0.183 |
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| 12.5 |
0.151 |
0.154 |
0.153 |
0.060 |
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| 0 |
0.093 |
0.092 |
0.093 |
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货期
3-5 working days