产品描述

This Human APEX1 ELISA Kit was designed for the quantitative measurement of Human APEX1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

AP endonuclease 1 ELISA Kit; AP endonuclease class I ELISA Kit; AP lyase ELISA Kit; APE 1 ELISA Kit; APE ELISA Kit; APE-1 ELISA Kit; APEN ELISA Kit; APEX 1 ELISA Kit; APEX ELISA Kit; APEX nuclease (multifunctional DNA repair enzyme) 1 ELISA Kit; Apex nuclease 1 ELISA Kit; APEX nuclease ELISA Kit; APEX1 ELISA Kit; APEX1_HUMAN ELISA Kit; Apurinic endonuclease ELISA Kit; Apurinic-apyrimidinic endonuclease 1 ELISA Kit; Apurinic/apyrimidinic (abasic) endonuclease ELISA Kit; Apurinic/apyrimidinic endonuclease 1 ELISA Kit; Apurinic/apyrimidinic exonuclease ELISA Kit; APX ELISA Kit; BAP1 ELISA Kit; Deoxyribonuclease (apurinic or apyrimidinic) ELISA Kit; DNA (apurinic or apyrimidinic site) lyase ELISA Kit; DNA-(apurinic or apyrimidinic site) lyase; mitochondrial ELISA Kit; EC 4.2.99.18 ELISA Kit; HAP 1 ELISA Kit; HAP1 ELISA Kit; Human Apurinic endonuclease 1 ELISA Kit; MGC139790 ELISA Kit; Multifunctional DNA repair enzyme ELISA Kit; Redox factor 1 ELISA Kit; Redox factor-1 ELISA Kit; REF 1 ELISA Kit; REF 1 protein ELISA Kit; REF-1 ELISA Kit; REF1 ELISA Kit; REF1 protein ELISA Kit

缩写

APEX1

Uniprot No

P27695

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human APEX1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		104
		Range %		93-108
1:2		Average %		104
		Range %		95-107
1:4		Average %		91
		Range %		84-95
1:8		Average %		92
		Range %		84-95

回收率

The recovery of human APEX1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		100		89-105
EDTA plasma (n=4)		104		93-108

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 1000 2.253 2.155 2.204 2.058 500 1.398 1.434 1.416 1.270 250 0.916 0.900 0.908 0.762 125 0.507 0.487 0.497 0.351 62.5 0.351 0.366 0.359 0.213 31.25 0.268 0.272 0.270 0.124 15.6 0.229 0.234 0.232 0.086 0 0.147 0.145 0.146

货期

3-5 working days