产品描述

This Human CLEC7A ELISA Kit was designed for the quantitative measurement of Human CLEC7A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

别名

Beta GR ELISA Kit; Beta-glucan receptor ELISA Kit; BGR ELISA Kit; C type calcium dependent carbohydrate recognition domain lectin superfamily member 12 ELISA Kit; C type lectin domain family 7 member a ELISA Kit; C-type lectin domain family 7 member A ELISA Kit; C-type lectin superfamily member 12 ELISA Kit; CANDF4 ELISA Kit; CLC7A_HUMAN ELISA Kit; CLEC7A ELISA Kit; CLEC7A protein ELISA Kit; Clecsf12 ELISA Kit; DC-associated C-type lectin 1 ELISA Kit; Dectin 1 ELISA Kit; DECTIN 1 receptor ELISA Kit; Dectin-1 ELISA Kit; Dectin1 ELISA Kit; Dendritic cell associated C type lectin 1 ELISA Kit; Dendritic cell-associated C-type lectin 1 ELISA Kit; Lectin like receptor 1 ELISA Kit; Putative transmembrane protein dectin 1 ELISA Kit

缩写

CLEC7A

Uniprot No

Q9BXN2

灵敏度

7.81 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CLEC7A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

87

Range %

84-95

1:2

Average %

90

Range %

85-95

1:4

Average %

97

Range %

92-101

1:8

Average %

93

Range %

89-99

回收率

The recovery of human CLEC7A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type			Average % Recovery				Range
Serum (n=5)			100				96-104
EDTA plasma (n=4)			96				90-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 2.246 2.297 2.272 2.154 1000 1.821 1.862 1.842 1.724 500 1.315 1.336 1.326 1.208 250 0.887 0.874 0.881 0.763 125 0.453 0.463 0.458 0.340 62.5 0.322 0.342 0.332 0.214 31.25 0.262 0.254 0.258 0.140 0 0.119 0.117 0.118

货期

3-5 working days