产品描述
花生四烯酸 (AA) 是一种必需脂肪酸,在人体中起着至关重要的作用。它存在于许多食物中,包括鱼、蛋和肉。 AA 也由身体产生,对细胞生长和发育很重要。它与多种健康益处有关,包括改善心血管健康、改善认知功能和减少炎症。
华美生物所提供的Sheep arachidonic Acid(AA) ELISA kit属于ELISA检测试剂盒,采用竞争法定量检测绵羊 血清、血浆样本中的AA,其灵敏度为华美生物所提供的13.797 ng/mL,检测范围为15.625 ng/mL-1000 ng/mL。
缩写
AA
灵敏度
13.797 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Biochemicals
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep AA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
96 |
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| Range % |
89-100 |
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| 1:200 |
Average % |
90 |
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| Range % |
85-95 |
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| 1:400 |
Average % |
100 |
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| Range % |
94-104 |
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| 1:800 |
Average % |
105 |
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| Range % |
97-110 |
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回收率
| The recovery of sheep AA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
102 |
95-106 |
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| EDTA plasma (n=4) |
102 |
96-107 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
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| 1000 |
0.122 |
0.120 |
0.121 |
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| 500 |
0.166 |
0.158 |
0.162 |
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| 250 |
0.232 |
0.241 |
0.237 |
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| 125 |
0.337 |
0.344 |
0.341 |
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| 62.5 |
0.528 |
0.510 |
0.519 |
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| 31.25 |
0.941 |
0.955 |
0.948 |
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| 15.625 |
1.471 |
1.522 |
1.497 |
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| 0 |
2.326 |
2.419 |
2.373 |
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货期
3-5 working days