产品描述
This Guinea pig CCL2 ELISA Kit was designed for the quantitative measurement of Guinea pig CCL2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.
别名
CCL2 ELISA Kit; MCP1 ELISA Kit; SCYA2C-C motif chemokine 2 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Small-inducible cytokine A2 ELISA Kit
缩写
CCL2
Uniprot No
Q08782
灵敏度
5.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig MCP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 91 | |
| Range % | 86-95 | |
| 1:2 | Average % | 102 | |
| Range % | 97-107 | |
| 1:4 | Average % | 91 | |
| Range % | 85-97 | |
| 1:8 | Average % | 97 | |
| Range % | 91-103 | |
回收率
| The recovery of guinea pig MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-98 | |
| EDTA plasma (n=4) | 93 | 87-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1500 | 1.857 | 1.902 | 1.880 | 1.680 | | | 750 | 1.345 | 1.403 | 1.374 | 1.174 | | | 375 | 0.981 | 0.954 | 0.968 | 0.768 | | | 187 | 0.748 | 0.700 | 0.724 | 0.524 | | | 94 | 0.511 | 0.532 | 0.522 | 0.322 | | | 47 | 0.416 | 0.426 | 0.421 | 0.221 | | | 23.5 | 0.289 | 0.281 | 0.285 | 0.085 | | | 0 | 0.201 | 0.199 | 0.200 | | |
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货期
3-5 working days