产品描述
白蛋白(Albumin)是人体血液中最丰富的蛋白质之一,由肝脏合成并分泌到血液中。它具有多种重要的生物学功能,包括维持血浆渗透压和血容量、运输生物分子和药物、参与免疫调节和抗氧化等。此外,白蛋白还有助于组织修复和再生。由于其丰富的功能和广泛的应用价值,白蛋白在医学和生物科学研究中得到了广泛的关注和研究。
华美生物所提供的Pig Albumin (Alb) ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测猪血清、血浆、尿液样本中的ALB,其灵敏度为0.03 μg/mL,检测范围为0.03 μg/mL-7000 μg/mL。
别名
ALBSerum albumin ELISA Kit
缩写
ALB
Uniprot No
P08835
灵敏度
0.03 μg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig albumin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
90 |
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| Range % |
87-95 |
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| 1:200 |
Average % |
93 |
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| Range % |
90-100 |
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| 1:400 |
Average % |
90 |
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| Range % |
85-93 |
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| 1:800 |
Average % |
89 |
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| Range % |
82-96 |
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回收率
| The recovery of pig albumin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
93-101 |
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| EDTA plasma (n=4) |
93 |
89-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| μg/ml |
OD1 |
OD2 |
Average |
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| 7000 |
0.123 |
0.128 |
0.126 |
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| 875 |
0.327 |
0.311 |
0.319 |
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| 109.4 |
0.612 |
0.623 |
0.618 |
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| 13.7 |
0.837 |
0.845 |
0.841 |
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| 1.7 |
0.950 |
0.967 |
0.959 |
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| 0.2 |
1.236 |
1.221 |
1.229 |
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| 0.03 |
1.493 |
1.518 |
1.506 |
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| 0 |
1.844 |
1.904 |
1.874 |
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货期
3-5 working days