产品描述
This Mouse APH1B ELISA Kit was designed for the quantitative measurement of Mouse APH1B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.
别名
Aph1b; Gamma-secretase subunit APH-1B; APH-1b; Aph-1beta
缩写
APH1B
Uniprot No
Q8C7N7
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse γ-Secretase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 90-102 | |
| 1:2 | Average % | 102 | |
| Range % | 97-107 | |
| 1:4 | Average % | 89 | |
| Range % | 87-93 | |
| 1:8 | Average % | 94 | |
| Range % | 90-98 | |
回收率
| The recovery of mouse γ-Secretase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 103 | 99-106 | |
| EDTA plasma (n=4) | 95 | 89-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.234 | 2.356 | 2.295 | 2.130 | | | 250 | 1.367 | 1.378 | 1.373 | 1.208 | | | 125 | 0.810 | 0.824 | 0.817 | 0.652 | | | 62.5 | 0.492 | 0.452 | 0.472 | 0.307 | | | 31.2 | 0.313 | 0.306 | 0.310 | 0.145 | | | 15.6 | 0.236 | 0.250 | 0.243 | 0.078 | | | 7.8 | 0.205 | 0.209 | 0.207 | 0.042 | | | 0 | 0.160 | 0.169 | 0.165 | | |
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货期
3-5 working days