产品描述

The human AGTR1 ELISA kit is a solid-phase immunoassay specially designed to quantitatively measure human AGTR1 in serum, plasma, tissue homogenates, or cell lysates. It is based on the Sandwich-ELISA mechanism. AGTR1 in the sample is bound to the capture antibody immobilized on the 96-well strip plate and then sandwiched with the biotinylated AGTR1 antibody. After the addition of HRP-avidin and TMB substrate, the solution in the wells turns blue. The color reaction is stopped by adding the stop solution into the wells, and the color changes from blue to yellow. The color intensity is positively proportional to the AGTR1 bound in the initial step. The AGTR1 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

Angiotensin II (ATII) interacts with AGTR1, which causes calcium-dependent phosphorylation of myosin, leading to vascular smooth muscle contraction. This arterial smooth muscle contraction is responsible for raising blood pressure. AGTR1 is mostly found in vascular smooth muscle cells, as well as the heart, adrenal gland, and kidney. It is involved in the etiology of hypertension and plays an important role in blood pressure management. The majority of evidence points to AGTR1 as a possible cause of pathologic conditions like hypertension. Angiotensin receptor blockers that target AGTR1 are routinely prescribed antihypertensive drugs that have been demonstrated to minimize the risk of other cardiovascular outcomes in addition to lowering blood pressure. As such, AGTR1 is an excellent candidate gene in the etiology of hypertension and other cardiovascular diseases (CVD).

别名

AG2S ELISA Kit; Agtr 1 ELISA Kit; Agtr1 ELISA Kit; AGTR1_HUMAN ELISA Kit; Agtr1a ELISA Kit; AGTR1B ELISA Kit; Ang II ELISA Kit; Angiotensin II receptor type 1 ELISA Kit; Angiotensin II type-1 receptor ELISA Kit; Angiotensin receptor 1 ELISA Kit; Angiotensin receptor 1B ELISA Kit; AT 1B ELISA Kit; AT 1r ELISA Kit; AT1 ELISA Kit; At1a ELISA Kit; AT1AR ELISA Kit; AT1B ELISA Kit; AT1BR ELISA Kit; AT1R ELISA Kit; AT2R1 ELISA Kit; AT2R1A ELISA Kit; AT2R1B ELISA Kit; HAT1R ELISA Kit; Type 1 angiotensin II receptor ELISA Kit; Type 1B angiotensin II receptor ELISA Kit; Type-1 angiotensin II receptor ELISA Kit

缩写

AGTR1

Uniprot No

P30556

灵敏度

11.72 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ANGⅡR-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		81-98
1:2		Average %		96
		Range %		93-105
1:4		Average %		94
		Range %		87-98
1:8		Average %		103
		Range %		96-110

回收率

The recovery of human ANGⅡR-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		87-96
EDTA plasma (n=4)		99		90-108

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 3000 2.354 2.254 2.304 2.153 1500 1.712 1.612 1.662 1.511 750 1.131 1.081 1.106 0.955 375 0.566 0.556 0.561 0.410 187.5 0.338 0.328 0.333 0.182 93.75 0.277 0.267 0.272 0.121 46.88 0.209 0.205 0.207 0.056 0 0.152 0.150 0.151

货期

3-5 working days