产品描述

This Human CAPN2 ELISA Kit was designed for the quantitative measurement of Human CAPN2 protein in serum, plasma, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

Calcium activated neutral proteinase 2 ELISA Kit; Calcium activated neutral proteinase ELISA Kit; Calcium-activated neutral protease 2; catalytic subunit ELISA Kit; Calcium-activated neutral proteinase 2 ELISA Kit; CALP80 ELISA Kit; Calpain 2 (m/II) large subunit ELISA Kit; Calpain 2 catalytic subunit ELISA Kit; Calpain 2 large catalytic subunit ELISA Kit; Calpain 2 large subunit ELISA Kit; Calpain 2; large [catalytic] subunit ELISA Kit; Calpain large polypeptide L2 ELISA Kit; Calpain M type ELISA Kit; Calpain M-type ELISA Kit; Calpain-2 catalytic subunit ELISA Kit; Calpain-2 large subunit ELISA Kit; Calpain2 ELISA Kit; CAN2_HUMAN ELISA Kit; CANP 2 ELISA Kit; CANP L2 ELISA Kit; CANP2 ELISA Kit; CANPL 2 ELISA Kit; CANPL2 ELISA Kit; CANPml ELISA Kit; Capa2 ELISA Kit; CAPN 2 ELISA Kit; CAPN2 ELISA Kit; FLJ39928 ELISA Kit; M calpain ELISA Kit; M calpin ELISA Kit; M type ELISA Kit; M-calpain ELISA Kit; mCANP ELISA Kit; Millimolar calpain ELISA Kit; Millimolar-calpain ELISA Kit

缩写

CAPN2

Uniprot No

P17655

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CAPN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		87
		Range %		84-90
1:2		Average %		104
		Range %		101-107
1:4		Average %		98
		Range %		94-102
1:8		Average %		101
		Range %		98-104

回收率

The recovery of human CAPN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		85		81-89
EDTA plasma (n=4)		92		88-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected     10 2.432 2.502 2.467 2.327     5 1.688 1.698 1.693 1.553     2.5 0.923 0.934 0.929 0.789     1.25 0.489 0.476 0.483 0.343     0.625 0.329 0.319 0.324 0.184     0.312 0.256 0.262 0.259 0.119     0.156 0.201 0.210 0.206 0.066     0 0.139 0.141 0.140

货期

3-5 working days