产品描述

This Human C10orf54 ELISA Kit was designed for the quantitative measurement of Human C10orf54 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

B7 H5 ELISA Kit; B7H5 ELISA Kit; C10orf54 ELISA Kit; chromosome 10 open reading frame 54 ELISA Kit; DD1alpha ELISA Kit; GI24 ELISA Kit; GI24_HUMAN ELISA Kit; PD-1H ELISA Kit; PDCD1 homolog ELISA Kit; Platelet receptor Gi24 ELISA Kit; PP2135 ELISA Kit; sisp 1 ELISA Kit; SISP1 ELISA Kit; stress induced secreted protein 1 ELISA Kit; UNQ730/PRO1412 ELISA Kit; V domain Ig suppressor of T cell activation ELISA Kit; V set domain containing immunoregulatory receptor ELISA Kit; V set immunoregulatory receptor ELISA Kit; V type immunoglobulin domain containing suppressor of T cell activation ELISA Kit; VISTA ELISA Kit

缩写

C10orf54

Uniprot No

Q9H7M9

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human C10orf54 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		85
		Range %		83-89
1:2		Average %		100
		Range %		95-105
1:4		Average %		84
		Range %		81-89
1:8		Average %		92
		Range %		88-96

回收率

The recovery of human C10orf54 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		101		98-104
EDTA plasma (n=4)		99		95-103

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 10 2.366 2.305 2.336 2.213 5 1.642 1.658 1.650 1.527 2.5 0.898 0.868 0.883 0.760 1.25 0.475 0.455 0.465 0.342 0.625 0.320 0.326 0.323 0.200 0.312 0.249 0.251 0.250 0.127 0.156 0.195 0.193 0.194 0.071 0 0.125 0.120 0.123

货期

3-5 working days