产品描述
This Rabbit BGLAP ELISA Kit was designed for the quantitative measurement of Rabbit BGLAP protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 1.25 ng/mL-80 ng/mL and the sensitivity is 0.31 ng/mL.
别名
BGLAP ELISA Kit; Osteocalcin ELISA Kit; Bone Gla protein ELISA Kit; BGP ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit
缩写
BGLAP
Uniprot No
P39056
灵敏度
0.31 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit OT/BGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 82-93 | |
| 1:2 | Average % | 95 | |
| Range % | 89-99 | |
| 1:4 | Average % | 94 | |
| Range % | 90-90 | |
| 1:8 | Average % | 94 | |
| Range % | 86-98 | |
回收率
| The recovery of rabbit OT/BGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 90-94 | |
| EDTA plasma (n=4) | 92 | 87-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 80 | 2.780 | 2.701 | 2.741 | 2.641 | | | 40 | 2.124 | 2.056 | 2.090 | 1.990 | | | 20 | 1.266 | 1.234 | 1.250 | 1.150 | | | 10 | 0.667 | 0.621 | 0.644 | 0.544 | | | 5 | 0.353 | 0.335 | 0.344 | 0.244 | | | 2.5 | 0.262 | 0.249 | 0.256 | 0.156 | | | 1.25 | 0.219 | 0.208 | 0.214 | 0.114 | | | 0 | 0.102 | 0.098 | 0.100 | | |
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货期
3-5 working days