产品描述
This Human 6ketoPGF1a ELISA Kit was designed for the quantitative measurement of Human 6ketoPGF1a protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 50 pg/mL-800 pg/mL and the sensitivity is 50 pg/mL.
缩写
6ketoPGF1a
灵敏度
50 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human 6-K-PGF1a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
87-99 |
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| 1:2 |
Average % |
97 |
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| Range % |
93-105 |
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| 1:4 |
Average % |
85 |
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| Range % |
82-89 |
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| 1:8 |
Average % |
90 |
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| Range % |
85-98 |
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回收率
| The recovery of human 6-K-PGF1a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
85-95 |
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| EDTA plasma (n=4) |
95 |
89-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 800 |
0.218 |
0.211 |
0.215 |
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| 400 |
0.350 |
0.333 |
0.342 |
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| 200 |
0.603 |
0.612 |
0.608 |
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| 100 |
1.111 |
1.102 |
1.107 |
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| 50 |
2.219 |
2.178 |
2.199 |
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货期
3-5 working days