产品描述
This Mouse apo-B48 ELISA Kit was designed for the quantitative measurement of Mouse apo-B48 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 62.5 ng/mL-4000 ng/mL and the sensitivity is 15.6 ng/mL.
缩写
apo-B48
灵敏度
15.6 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse apo-B48 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:200 |
Average % |
96 |
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| Range % |
92-104 |
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| 1:400 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:800 |
Average % |
87 |
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| Range % |
84-92 |
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| 1:1600 |
Average % |
87 |
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| Range % |
82-93 |
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回收率
| The recovery of mouse apo-B48 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-99 |
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| EDTA plasma (n=4) |
98 |
94-102 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 4000 |
0.073 |
0.074 |
0.074 |
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| 2000 |
0.117 |
0.120 |
0.119 |
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| 1000 |
0.183 |
0.192 |
0.188 |
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| 500 |
0.283 |
0.275 |
0.279 |
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| 250 |
0.451 |
0.448 |
0.450 |
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| 125 |
0.770 |
0.768 |
0.769 |
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| 62.5 |
1.048 |
1.122 |
1.085 |
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| 0 |
2.275 |
2.371 |
2.323 |
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货期
3-5 working days