产品描述
This Pig C-Peptide ELISA Kit was designed for the quantitative measurement of Pig C-Peptide protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.25 ng/mL-80 ng/mL and the sensitivity is 0.312 ng/mL .
缩写
C-Peptide
灵敏度
0.312 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig C-Peptide in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 94 | |
| Range % | 88-98 | |
| 1:2 | Average % | 96 | |
| Range % | 91-102 | |
| 1:4 | Average % | 103 | |
| Range % | 97-107 | |
| 1:8 | Average % | 87 | |
| Range % | 81-93 | |
回收率
| The recovery of pig C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 93-104 | |
| EDTA plasma (n=4) | 92 | 87-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | 80 | 2.663 | 2.562 | 2.613 | 2.487 | | | 40 | 2.093 | 2.214 | 2.154 | 2.028 | | | 20 | 1.510 | 1.531 | 1.521 | 1.395 | | | 10 | 1.043 | 1.074 | 1.059 | 0.933 | | | 5 | 0.634 | 0.623 | 0.629 | 0.503 | | | 2.5 | 0.381 | 0.370 | 0.376 | 0.250 | | | 1.25 | 0.248 | 0.244 | 0.246 | 0.120 | | | 0 | 0.127 | 0.125 | 0.126 | | |
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货期
3-5 working days