产品描述
This Rat Casp-9 ELISA Kit was designed for the quantitative measurement of Rat Casp-9 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.
缩写
Casp-9
Uniprot No
Q9JHK1
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 90-99 | |
| 1:2 | Average % | 95 | |
| Range % | 91-102 | |
| 1:4 | Average % | 98 | |
| Range % | 94-103 | |
| 1:8 | Average % | 102 | |
| Range % | 96-106 | |
回收率
| The recovery of rat Casp-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 89-98 | |
| EDTA plasma (n=4) | 100 | 96-104 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 4000 | 1.846 | 1.946 | 1.896 | 1.752 | | | 2000 | 1.442 | 1.542 | 1.492 | 1.348 | | | 1000 | 0.986 | 1.016 | 1.001 | 0.857 | | | 500 | 0.546 | 0.556 | 0.551 | 0.407 | | | 250 | 0.320 | 0.330 | 0.325 | 0.181 | | | 125 | 0.266 | 0.278 | 0.272 | 0.128 | | | 62.5 | 0.204 | 0.207 | 0.206 | 0.062 | | | 0 | 0.143 | 0.144 | 0.144 | | |
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货期
3-5 working days