产品描述
This Rat Col Ⅰ ELISA Kit was designed for the quantitative measurement of Rat Col Ⅰ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL .
别名
Collagen Type 1,Col 1,_x000D_
Collagen Type I,Col I
缩写
Col Ⅰ
灵敏度
19.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
80-88 |
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| 1:2 |
Average % |
89 |
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| Range % |
84-93 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-96 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-99 |
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回收率
| The recovery of rat Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
88-98 |
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| EDTA plasma (n=4) |
98 |
93-103 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 5000 |
2.701 |
2.762 |
2.732 |
2.537 |
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| 2500 |
1.903 |
1.954 |
1.929 |
1.734 |
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| 1250 |
1.195 |
1.207 |
1.201 |
1.006 |
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| 625 |
0.802 |
0.743 |
0.773 |
0.578 |
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| 312 |
0.489 |
0.466 |
0.478 |
0.283 |
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| 156 |
0.376 |
0.359 |
0.368 |
0.173 |
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| 78 |
0.258 |
0.243 |
0.251 |
0.056 |
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| 0 |
0.201 |
0.189 |
0.195 |
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货期
3-5 working days