产品描述
This Human AREG ELISA Kit was designed for the quantitative measurement of Human AREG protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 4.7 pg/mL-300 pg/mL and the sensitivity is 1.1 pg/mL.
别名
AREG; AREGB; SDGF; Amphiregulin; AR; Colorectum cell-derived growth factor; CRDGF
缩写
AREG
Uniprot No
P15514
灵敏度
1.1 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human KAF/AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 93 | |
| Range % | 86-97 | |
| 1:2 | Average % | 83 | |
| Range % | 80-86 | |
| 1:4 | Average % | 91 | |
| Range % | 85-97 | |
| 1:8 | Average % | 98 | |
| Range % | 95-101 | |
回收率
| The recovery of human KAF/AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 85-93 | |
| EDTA plasma (n=4) | 104 | 100-108 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 300 | 2.812 | 2.836 | 2.824 | 2.681 | | | 150 | 1.971 | 1.965 | 1.968 | 1.825 | | | 75 | 1.122 | 1.168 | 1.145 | 1.002 | | | 37.5 | 0.590 | 0.571 | 0.581 | 0.438 | | | 18.8 | 0.385 | 0.399 | 0.392 | 0.249 | | | 9.4 | 0.293 | 0.273 | 0.283 | 0.140 | | | 4.7 | 0.210 | 0.216 | 0.213 | 0.070 | | | 0 | 0.142 | 0.144 | 0.143 | | |
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货期
3-5 working days