产品描述
This Mouse Col Ⅲ ELISA Kit was designed for the quantitative measurement of Mouse Col Ⅲ protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.
别名
Collagen Type 3,Col 3,_x000D_
Collagen Type III,Col III
缩写
Col Ⅲ
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:20 |
Average % |
89 |
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| Range % |
82-96 |
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| 1:40 |
Average % |
98 |
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| Range % |
94-102 |
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| 1:80 |
Average % |
97 |
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| Range % |
90-104 |
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| 1:160 |
Average % |
86 |
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| Range % |
82-90 |
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回收率
| The recovery of mouse Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
87-99 |
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| EDTA plasma (n=4) |
100 |
96-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.553 |
2.546 |
2.550 |
2.357 |
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| 100 |
1.875 |
1.954 |
1.915 |
1.722 |
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| 50 |
1.297 |
1.306 |
1.302 |
1.109 |
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| 25 |
0.832 |
0.875 |
0.854 |
0.661 |
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| 12.5 |
0.513 |
0.556 |
0.535 |
0.342 |
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| 6.25 |
0.375 |
0.395 |
0.385 |
0.192 |
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| 3.12 |
0.246 |
0.253 |
0.250 |
0.057 |
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| 0 |
0.188 |
0.197 |
0.193 |
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货期
3-5 working days