产品描述

The ELISA Kit is designed to measure human BACE1 levels in samples, including serum, plasma, or tissue homogenates. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of BACE1 in samples. The color intensity is measured at 450 nm via a microplate reader.

BACE1 is an aspartic protease that catalyzes the rate-limiting, initial cleavage at the β site of amyloid precursor protein (APP) and is followed by sequential intra-membrane processing at multiple sites by γ-secretase in the production of amyloid-β (Aβ). It is widely expressed in the brain, particularly in neurons, oligodendrocytes, and astrocytes, with particular abundance in various neuronal cell types. Inhibition of BACE1 proteolytic activity has been demonstrated to reduce Aβ generation and amyloid deposition, and thus specific inhibition of BACE1 by small molecules is a current focus for Alzheimer's disease therapy. In addition to the amyloidogenic pathway, BACE1 is involved in synaptic plasticity and synaptic homeostasis.

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These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   200 2.168 2.052 2.110 2.017   100 1.907 1.860 1.884 1.791   50 1.595 1.494 1.545 1.452   25 1.324 1.239 1.282 1.189   12.5 0.906 0.874 0.890 0.797   6.25 0.429 0.409 0.419 0.326   3.12 0.277 0.269 0.273 0.180   0 0.096 0.090 0.093

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