产品描述
This Human BMP2 ELISA Kit was designed for the quantitative measurement of Human BMP2 protein in serum, urine, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL .
别名
BDA2 ELISA Kit; BMP-2 ELISA Kit; BMP-2A ELISA Kit; Bmp2 ELISA Kit; BMP2_HUMAN ELISA Kit; BMP2A ELISA Kit; Bone morphogenetic protein 2 ELISA Kit; Bone morphogenetic protein 2A ELISA Kit
缩写
BMP2
Uniprot No
P12643
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human BMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 80-95 | |
| 1:2 | Average % | 95 | |
| Range % | 91-99 | |
| 1:4 | Average % | 97 | |
| Range % | 92-106 | |
| 1:8 | Average % | 93 | |
| Range % | 86-99 | |
回收率
| The recovery of human BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 4000 | 2.673 | 2.786 | 2.730 | 2.572 | | | 2000 | 2.345 | 2.324 | 2.335 | 2.177 | | | 1000 | 1.639 | 1.740 | 1.690 | 1.532 | | | 500 | 1.180 | 1.200 | 1.190 | 1.032 | | | 250 | 0.831 | 0.879 | 0.855 | 0.697 | | | 125 | 0.592 | 0.605 | 0.599 | 0.441 | | | 62.5 | 0.345 | 0.356 | 0.351 | 0.193 | | | 0 | 0.154 | 0.162 | 0.158 | | |
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货期
3-5 working days