产品描述
CSF2是一种胞外分泌蛋白,也被称为粒细胞-巨噬细胞集落刺激因子(GM-CSF),它是细胞因子家族的一员。CSF2在免疫调节、炎症反应和造血过程中发挥重要作用,它能促进白细胞增殖和分化,增强嗜中性粒细胞、单核细胞和巨噬细胞的活性,从而增强免疫反应。此外,CSF2还参与了许多疾病的发病机制,包括哮喘、肺部感染、类风湿性关节炎等。
华美生物所提供的Human Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、细胞培养上清、组织匀浆样本中的CSF2,其灵敏度为3.9 pg/mL,检测范围为15.6 pg/mL-1000 pg/mL。
别名
Colony stimulating factor 2 (granulocyte-macrophage) ELISA Kit; Colony Stimulating Factor 2 ELISA Kit; Colony stimulating factor ELISA Kit; Colony-stimulating factor ELISA Kit; CSF 2 ELISA Kit; CSF ELISA Kit; CSF2 ELISA Kit; CSF2_HUMAN ELISA Kit; GM-CSF ELISA Kit; GMCSF ELISA Kit; Granulocyte Macrophage Colony Stimulating Factor ELISA Kit; Granulocyte-macrophage colony-stimulating factor ELISA Kit; MGC131935 ELISA Kit; MGC138897 ELISA Kit; MGI1GM ELISA Kit; Molgramostin ELISA Kit; Pluripoietin-a ELISA Kit; Sargramostim ELISA Kit
缩写
CSF2
Uniprot No
P04141
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human GM-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
82-99 |
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| 1:2 |
Average % |
95 |
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| Range % |
88-102 |
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| 1:4 |
Average % |
95 |
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| Range % |
86-99 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-105 |
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回收率
| The recovery of human GM-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
86-100 |
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| EDTA plasma (n=4) |
96 |
90-110 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.987 |
2.913 |
2.950 |
2.792 |
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| 500 |
2.417 |
2.429 |
2.423 |
2.265 |
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| 250 |
1.674 |
1.686 |
1.680 |
1.522 |
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| 125 |
0.954 |
0.963 |
0.959 |
0.801 |
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| 62.5 |
0.576 |
0.562 |
0.569 |
0.411 |
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| 31.2 |
0.386 |
0.394 |
0.390 |
0.232 |
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| 15.6 |
0.261 |
0.268 |
0.265 |
0.107 |
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| 0 |
0.156 |
0.159 |
0.158 |
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货期
3-5 working days