产品描述

This Human BMPR1A ELISA Kit was designed for the quantitative measurement of Human BMPR1A protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

别名

10q23del ELISA Kit; Activin A receptor type II like kinase 3 ELISA Kit; Activin receptor like kinase 3 ELISA Kit; Activin receptor-like kinase 3 ELISA Kit; ACVRLK 3 ELISA Kit; ACVRLK3 ELISA Kit; ALK 3 ELISA Kit; ALK-3 ELISA Kit; ALK3 ELISA Kit; BMP type-1A receptor ELISA Kit; BMPR 1A ELISA Kit; Bmpr ELISA Kit; BMPR-1A ELISA Kit; Bmpr1a ELISA Kit; BMR1A_HUMAN ELISA Kit; Bone morphogenetic protein receptor type IA ELISA Kit; Bone morphogenetic protein receptor type IA precursor ELISA Kit; Bone morphogenetic protein receptor type-1A ELISA Kit; BR 1a ELISA Kit; BR1a ELISA Kit; CD 292 ELISA Kit; CD292 ELISA Kit; CD292 antigen ELISA Kit; EC 2.7.11.30 ELISA Kit; Serine threonine protein kinase receptor R5 ELISA Kit; Serine threonine protein kinase receptor R5 precursor ELISA Kit; Serine/threonine-protein kinase receptor R5 ELISA Kit; SKR 5 ELISA Kit; SKR5 ELISA Kit; zBMPR IA ELISA Kit; zBMPRIA ELISA Kit

缩写

BMPR1A

Uniprot No

P36894

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human BMPR-1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		86
		Range %		81-94
1:2		Average %		95
		Range %		87-98
1:4		Average %		95
		Range %		89-99
1:8		Average %		93
		Range %		86-97

回收率

The recovery of human BMPR-1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		91-102
EDTA plasma (n=4)		89		83-94

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 20 2.864 2.724 2.794 2.649 10 2.121 2.028 2.075 1.930 5 1.154 1.225 1.190 1.045 2.5 0.604 0.613 0.609 0.464 1.25 0.332 0.316 0.324 0.179 0.625 0.245 0.251 0.248 0.103 0.312 0.212 0.209 0.211 0.066 0 0.146 0.144 0.145

货期

3-5 working days