产品描述
The mouse BDNF ELISA Kit allows for the in vitro quantitative determination of BDNF concentrations in serum, plasma, cell culture supernates, or tissue homogenates. This kit exclusively recognizes mouse BDNF. BDNF is an important neurotrophic factor synthesized in the brain and ubiquitously distributed in the central nervous system (CNS) and peripheral nervous system. BDNF plays a prominent role in the neural structure and functional plasticity by promoting the survival, growth, differentiation, development of neurons, long-term potentiation, as well as regulation of synaptic transmission and activity. It is tightly associated with the pathogenesis of various diseases, including Alzheimer's disease, Parkinson's disease, and mood disorders. BDNF-TrkB signaling exerts roles in the CNS, especially in the cerebral cortex and hippocampus where it is involved in memory formation and long-term potentiation through the activation of several signaling pathways including MAPK/ERK, PLCg, and PI3K pathways.
The detection mechanism of this kit is based on the Sandwich-ELISA technique. BDNF in the sample is bound to the capture antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled BDNF antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with BDNF content in the sample. The kit has been quality-controlled with high sensitivity, strong specificity, good linearity, high precision, high recovery, and high lot-to-lot consistency.
别名
BdnfBrain-derived neurotrophic factor ELISA kit; BDNF) [Cleaved into: BDNF precursor form ELISA kit; ProBDNF)] ELISA kit
缩写
BDNF
Uniprot No
P21237
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse BDNF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
88-96 |
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| 1:2 |
Average % |
101 |
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| Range % |
97-105 |
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| 1:4 |
Average % |
96 |
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| Range % |
92-100 |
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| 1:8 |
Average % |
104 |
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| Range % |
99-109 |
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回收率
| The recovery of mouse BDNF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
87-101 |
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| EDTA plasma (n=4) |
88 |
81-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.420 |
2.355 |
2.388 |
2.251 |
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| 500 |
1.712 |
1.685 |
1.699 |
1.562 |
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| 250 |
0.984 |
0.994 |
0.989 |
0.852 |
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| 125 |
0.587 |
0.568 |
0.578 |
0.441 |
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| 62.5 |
0.393 |
0.377 |
0.385 |
0.248 |
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| 31.2 |
0.223 |
0.236 |
0.230 |
0.093 |
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| 7.8 |
0.150 |
0.160 |
0.155 |
0.018 |
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| 0 |
0.134 |
0.139 |
0.137 |
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货期
3-5 working days
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