产品描述

This Human ATP5B ELISA Kit was designed for the quantitative measurement of Human ATP5B protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

别名

ATP 5B ELISA Kit; ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide ELISA Kit; ATP synthase subunit beta mitochondrial ELISA Kit; ATP synthase subunit beta; mitochondrial ELISA Kit; atp5b ELISA Kit; ATPB ELISA Kit; ATPB_HUMAN ELISA Kit; ATPMB ELISA Kit; ATPSB ELISA Kit; Epididymis secretory protein Li 271 ELISA Kit; HEL-S-271 ELISA Kit; Mitochondrial ATP synthase beta subunit ELISA Kit; Mitochondrial ATP Synthase Subunit Beta ELISA Kit; Mitochondrial ATP synthetase beta subunit ELISA Kit

缩写

ATP5B

Uniprot No

P06576

灵敏度

6.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ATP5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		85
		Range %		81-89
1:2		Average %		96
		Range %		93-99
1:4		Average %		87
		Range %		84-90
1:8		Average %		87
		Range %		83-91

回收率

The recovery of human ATP5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		88-96
EDTA plasma (n=4)		96		93-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1600 2.308 2.356 2.332 2.191 800 1.597 1.558 1.578 1.437 400 0.919 0.938 0.929 0.788 200 0.549 0.587 0.568 0.427 100 0.405 0.434 0.420 0.279 50 0.293 0.272 0.283 0.142 25 0.201 0.205 0.203 0.062 0 0.141 0.140 0.141

货期

3-5 working days