产品描述

This Human ADAM33 ELISA Kit was designed for the quantitative measurement of Human ADAM33 protein in serum, cell culture supernates, saliva, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

别名

A disintegrin and metalloprotease 33 ELISA Kit; A disintegrin and metalloproteinase domain 33 ELISA Kit; ADA33_HUMAN ELISA Kit; ADAM 33 ELISA Kit; ADAM metallopeptidase domain 33 ELISA Kit; ADAM33 ELISA Kit; C20orf153 ELISA Kit; Disintegrin and metalloproteinase domain-containing protein 33 ELISA Kit; Disintegrin and reprolysin metalloproteinase family protein ELISA Kit; DJ964F7.1 ELISA Kit; DKFZp434K0521 ELISA Kit; FLJ35308 ELISA Kit; FLJ36751 ELISA Kit; Metalloprotease disintegrin ELISA Kit; MGC149823 ELISA Kit; MGC71889 ELISA Kit; PRO1891 ELISA Kit; UNQ873 ELISA Kit

缩写

ADAM33

Uniprot No

Q9BZ11

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ADAM33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		97
		Range %		92-101
1:2		Average %		89
		Range %		83-93
1:4		Average %		92
		Range %		88-96
1:8		Average %		95
		Range %		90-100

回收率

The recovery of human ADAM33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		93		89-97

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 2.748 2.672 2.710 2.581 1000 2.131 2.005 2.068 1.939 500 1.491 1.486 1.489 1.360 250 0.966 0.935 0.951 0.822 125 0.546 0.574 0.560 0.431 62.5 0.363 0.392 0.378 0.249 31.25 0.254 0.243 0.249 0.120 0 0.125 0.132 0.129

货期

3-5 working days