产品描述

This Human CCL25 ELISA Kit was designed for the quantitative measurement of Human CCL25 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

别名

A130072A22Rik ELISA Kit; C-C motif chemokine 25 ELISA Kit; CCL 25 ELISA Kit; CCL25 ELISA Kit; CCL25_HUMAN ELISA Kit; Chemokine (C C motif) ligand 25 ELISA Kit; Chemokine (CC motif) ligand 25 ELISA Kit; Chemokine C C motif ligand 25 ELISA Kit; Chemokine CC motif ligand 25 ELISA Kit; Chemokine TECK ELISA Kit; Ck beta 15 ELISA Kit; Ckb 15 ELISA Kit; Ckb15 ELISA Kit; MGC125074 ELISA Kit; MGC150327 ELISA Kit; SCY A25 ELISA Kit; SCYA 25 ELISA Kit; SCYA25 ELISA Kit; Small inducible cytokine A25 ELISA Kit; Small inducible cytokine A25 isoform 1 precursor ELISA Kit; Small inducible cytokine A25 isoform 2 precursor ELISA Kit; Small inducible cytokine subfamily A (Cys Cys) member 25 ELISA Kit; Small inducible cytokine subfamily A Cys Cys member 25 ELISA Kit; Small inducible cytokine subfamily A; member 25 ELISA Kit; Small-inducible cytokine A25 ELISA Kit; TECK ELISA Kit; TECKvar ELISA Kit; Thymus expressed chemokine ELISA Kit; Thymus-expressed chemokine ELISA Kit

缩写

CCL25

Uniprot No

O15444

灵敏度

6.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TECK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		87-98
1:2		Average %		100
		Range %		96-104
1:4		Average %		89
		Range %		84-94
1:8		Average %		95
		Range %		89-100

回收率

The recovery of human TECK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		85-97
EDTA plasma (n=4)		104		97-110

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 1600 2.684 2.564 2.624 2.498 800 2.011 1.937 1.974 1.848 400 1.374 1.339 1.357 1.231 200 0.945 0.908 0.927 0.801 100 0.528 0.516 0.522 0.396 50 0.320 0.307 0.314 0.188 25 0.238 0.231 0.235 0.109 0 0.129 0.123 0.126

货期

3-5 working days