产品描述
8-OHdG,也称为 8-羟基脱氧鸟苷,是体内氧化应激的标志物。它是一种生物标志物,可用于评估氧化损伤的水平和识别潜在的健康问题。 8-OHdG 已被发现在患有某些疾病(如癌症、糖尿病、心血管疾病和神经退行性疾病)的个体中升高。此外,它还与衰老和空气污染等环境压力因素有关。研究表明,测量 8-OHdG 水平可以帮助诊断疾病状态和监测治疗效果。因此,它是临床医学中评估患者氧化应激的重要工具。
华美生物所提供的Rat 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、血浆、细胞培养上清、组织匀浆样本中的8-OHdG,其灵敏度为华美生物所提供的0.078 ng/mL,检测范围为0.312 ng/mL-20 ng/mL。
缩写
8-OHdG
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
88-98 |
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| 1:2 |
Average % |
96 |
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| Range % |
91-102 |
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| 1:4 |
Average % |
90 |
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| Range % |
85-96 |
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| 1:8 |
Average % |
96 |
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| Range % |
88-99 |
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回收率
| The recovery of rat 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
102 |
98-107 |
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| EDTA plasma (n=4) |
96 |
90-102 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
1.890 |
1.780 |
1.835 |
1.682 |
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| 10 |
1.198 |
1.128 |
1.163 |
1.010 |
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| 5 |
0.756 |
0.726 |
0.741 |
0.588 |
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| 2.5 |
0.525 |
0.495 |
0.510 |
0.357 |
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| 1.25 |
0.321 |
0.318 |
0.320 |
0.167 |
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| 0.625 |
0.272 |
0.262 |
0.267 |
0.114 |
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| 0.312 |
0.213 |
0.209 |
0.211 |
0.058 |
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| 0 |
0.154 |
0.152 |
0.153 |
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货期
3-5 working days