产品描述
This Human CD276 ELISA Kit was designed for the quantitative measurement of Human CD276 protein in serum, plasma, urine, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL .
别名
4Ig B7 H3 ELISA Kit; 4Ig-B7-H3 ELISA Kit; AU016588 ELISA Kit; B7 H3 ELISA Kit; B7 homolog 3 ELISA Kit; B7-H3 ELISA Kit; B7H3 ELISA Kit; B7RP-2 ELISA Kit; CD_antigen=CD276 ELISA Kit; CD276 ELISA Kit; CD276 antigen ELISA Kit; CD276 molecule ELISA Kit; CD276_HUMAN ELISA Kit; Costimulatory molecule ELISA Kit; Flags: Precursor ELISA Kit; PSEC0249 ELISA Kit; UNQ309/PRO352 ELISA Kit
缩写
CD276
Uniprot No
Q5ZPR3
灵敏度
0.78 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human sCD276/sB7-H3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 88 | |
| Range % | 84-92 | |
| 1:2 | Average % | 94 | |
| Range % | 90-98 | |
| 1:4 | Average % | 99 | |
| Range % | 95-104 | |
| 1:8 | Average % | 102 | |
| Range % | 96-108 | |
回收率
| The recovery of human sCD276/sB7-H3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 93-96 | |
| EDTA plasma (n=4) | 100 | 95-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 200 | 2.398 | 2.387 | 2.393 | 2.240 | | | 100 | 1.974 | 1.999 | 1.987 | 1.834 | | | 50 | 1.379 | 1.398 | 1.389 | 1.236 | | | 25 | 0.854 | 0.801 | 0.828 | 0.675 | | | 12.5 | 0.486 | 0.472 | 0.479 | 0.326 | | | 6.25 | 0.335 | 0.325 | 0.330 | 0.177 | | | 3.12 | 0.262 | 0.279 | 0.271 | 0.118 | | | 0 | 0.152 | 0.154 | 0.153 | | |
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货期
3-5 working days