产品描述
This Rat ANGPT1 ELISA Kit was designed for the quantitative measurement of Rat ANGPT1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.
别名
Angpt1Angiopoietin-1 ELISA kit; ANG-1 ELISA kit
缩写
ANGPT1
Uniprot No
O35460
灵敏度
1.56 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ANG-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 104 | | |
| Range % | 93-109 | | |
| 1:2 | Average % | 112 | | |
| Range % | 103-116 | | |
| 1:4 | Average % | 94 | | |
| Range % | 84-98 | | |
| 1:8 | Average % | 90 | | |
| Range % | 83-94 | | |
回收率
| The recovery of rat ANG-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 90 | 83-94 | | |
| EDTA plasma (n=4) | 103 | 92-108 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | | 400 | 2.985 | 2.845 | 2.915 | 2.781 | | | | 200 | 2.681 | 2.579 | 2.630 | 2.496 | | | | 100 | 2.297 | 2.213 | 2.255 | 2.121 | | | | 50 | 1.684 | 1.622 | 1.653 | 1.519 | | | | 25 | 1.001 | 0.956 | 0.979 | 0.845 | | | | 12.5 | 0.449 | 0.441 | 0.445 | 0.311 | | | | 6.25 | 0.251 | 0.242 | 0.247 | 0.113 | | | | 0 | 0.132 | 0.136 | 0.134 | | | |
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货期
3-5 working days