产品描述
This Mouse 6ketoPGF1a ELISA Kit was designed for the quantitative measurement of Mouse 6ketoPGF1a protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 25 pg/mL-1000 pg/mL and the sensitivity is 25 pg/mL.
缩写
6ketoPGF1a
灵敏度
25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse 6-K-PGF1a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
86-109 |
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| 1:2 |
Average % |
92 |
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| Range % |
86-105 |
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| 1:4 |
Average % |
90 |
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| Range % |
85-105 |
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| 1:8 |
Average % |
93 |
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| Range % |
84-100 |
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回收率
| The recovery of mouse 6-K-PGF1a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
86-107 |
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| EDTA plasma (n=4) |
94 |
83-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 1000 |
0.130 |
0.136 |
0.133 |
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| 500 |
0.234 |
0.220 |
0.227 |
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| 230 |
0.359 |
0.343 |
0.351 |
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| 75 |
0.618 |
0.594 |
0.606 |
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| 25 |
1.204 |
1.227 |
1.216 |
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| 0 |
2.592 |
2.625 |
2.609 |
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货期
3-5 working days