产品描述
This Human ARL8B ELISA Kit was designed for the quantitative measurement of Human ARL8B protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .
别名
ADP ribosylation factor like 10C ELISA Kit; ADP ribosylation factor like 8B ELISA Kit; ADP-ribosylation factor-like protein 10C ELISA Kit; ADP-ribosylation factor-like protein 8B ELISA Kit; ARL10C ELISA Kit; ARL8B ELISA Kit; ARL8B_HUMAN ELISA Kit; FLJ10702 ELISA Kit; Gie1 ELISA Kit; Novel small G protein indispensable for equal chromosome segregation 1 ELISA Kit
缩写
ARL8B
Uniprot No
Q9NVJ2
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Epigenetics and Nuclear Signaling
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human ARL8B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
96 |
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| Range % |
90-100 |
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| 1:10 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:20 |
Average % |
104 |
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| Range % |
99-109 |
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| 1:40 |
Average % |
84 |
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| Range % |
80-88 |
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回收率
| The recovery of human ARL8B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
95-104 |
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| EDTA plasma (n=4) |
90 |
85-94 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.680 |
2.752 |
2.716 |
2.538 |
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| 500 |
1.979 |
2.076 |
2.028 |
1.850 |
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| 250 |
1.230 |
1.214 |
1.222 |
1.044 |
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| 125 |
0.773 |
0.739 |
0.756 |
0.578 |
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| 62.5 |
0.455 |
0.476 |
0.466 |
0.288 |
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| 31.2 |
0.384 |
0.369 |
0.377 |
0.199 |
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| 15.6 |
0.270 |
0.286 |
0.278 |
0.100 |
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| 0 |
0.175 |
0.181 |
0.178 |
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货期
3-5 working days