产品描述

This Human ACTB ELISA Kit was designed for the quantitative measurement of Human ACTB protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

别名

A26C1A ELISA Kit; A26C1B ELISA Kit; ACTB ELISA Kit; ACTB_HUMAN ELISA Kit; Actin beta ELISA Kit; Actin cytoplasmic 1 ELISA Kit; Actin; cytoplasmic 1; N-terminally processed ELISA Kit; Actx ELISA Kit; b actin ELISA Kit; b-actin ELISA Kit; Beta cytoskeletal actin ELISA Kit; Beta-actin ELISA Kit; BRWS1 ELISA Kit; E430023M04Rik ELISA Kit; Melanoma X actin ELISA Kit; MGC128179 ELISA Kit; PS1TP5 binding protein 1 ELISA Kit; PS1TP5BP1 ELISA Kit

缩写

ACTB

Uniprot No

P60709

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human β-actin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		80-105
1:2		Average %		92
		Range %		85-100
1:4		Average %		97
		Range %		92-105
1:8		Average %		90
		Range %		83-100

回收率

The recovery of human β-actin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		82-100
EDTA plasma (n=4)		95		89-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 20 2.652 2.641 2.647 2.497 10 2.134 1.993 2.064 1.914 5 1.548 1.486 1.517 1.367 2.5 0.987 0.960 0.974 0.824 1.25 0.595 0.578 0.587 0.437 0.625 0.402 0.397 0.400 0.250 0.312 0.263 0.245 0.254 0.104 0 0.154 0.145 0.150

货期

3-5 working days