产品描述

This Human COLEC12 ELISA Kit was designed for the quantitative measurement of Human COLEC12 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

别名

CL P1 ELISA Kit; CL-P1 ELISA Kit; CLP1 ELISA Kit; COL12_HUMAN ELISA Kit; COLEC 12 ELISA Kit; colec12 ELISA Kit; Collectin placenta 1 ELISA Kit; Collectin placenta protein 1 ELISA Kit; Collectin sub family member 12 ELISA Kit; Collectin-12 ELISA Kit; hCL P1 ELISA Kit; hCL-P1 ELISA Kit; NSR2 ELISA Kit; Nurse cell scavenger receptor 2 ELISA Kit; SCARA4 ELISA Kit; Scavenger receptor class A member 4 ELISA Kit; Scavenger receptor with C type lectin ELISA Kit; Scavenger receptor with C-type lectin ELISA Kit; SRCL ELISA Kit

缩写

COLEC12

Uniprot No

Q5KU26

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human COLEC12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		84
		Range %		80-88
1:2		Average %		85
		Range %		82-89
1:4		Average %		91
		Range %		86-95
1:8		Average %		104
		Range %		101-107

回收率

The recovery of human COLEC12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		89		85-93
EDTA plasma (n=4)		90		85-95

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 20 2.541 2.698 2.620 2.498 10 2.039 2.000 2.020 1.898 5 1.307 1.368 1.338 1.216 2.5 0.717 0.738 0.728 0.606 1.25 0.402 0.398 0.400 0.278 0.625 0.281 0.278 0.280 0.158 0.312 0.183 0.179 0.181 0.059 0 0.123 0.120 0.122

货期

3-5 working days