产品描述
CEACAM5是一种糖蛋白,其编码基因位于人类基因组上的19号染色体上。CEACAM5在许多类型的肿瘤中都过表达,因此被认为是一种癌症标志物。它在细胞外表面上发挥着多种生物学作用,包括细胞黏附、信号转导和免疫调节。
华美生物所提供的Human carcinoembryonic antigen,CEA ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的CEACAM5,其灵敏度为2 ng/mL,检测范围为5 ng/mL-120 ng/mL。
别名
Carcinoembryonic antigen ELISA Kit; Carcinoembryonic antigen-related cell adhesion molecule 5 ELISA Kit; CD66e ELISA Kit; CEA ELISA Kit; Ceacam5 ELISA Kit; CEAM5_HUMAN ELISA Kit; DKFZp781M2392 ELISA Kit; Meconium antigen 100 ELISA Kit; OTTHUMP00000199032 ELISA Kit; OTTHUMP00000199033 ELISA Kit; OTTHUMP00000199034 ELISA Kit
缩写
CEACAM5
Uniprot No
P06731
灵敏度
2 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<10% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<15% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
98 |
|
| Range % |
92-106 |
|
| 1:2 |
Average % |
95 |
|
| Range % |
91-99 |
|
| 1:4 |
Average % |
104 |
|
| Range % |
98-114 |
|
| 1:8 |
Average % |
85 |
|
| Range % |
80-89 |
|
回收率
| The recovery of human CEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
88-98 |
|
| EDTA plasma (n=4) |
102 |
98-108 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 120 |
1.935 |
2.042 |
1.989 |
1.951 |
|
| 60 |
1.318 |
1.428 |
1.373 |
1.335 |
|
| 30 |
0.844 |
0.864 |
0.854 |
0.816 |
|
| 10 |
0.388 |
0.376 |
0.382 |
0.344 |
|
| 5 |
0.224 |
0.227 |
0.226 |
0.188 |
|
| 0 |
0.037 |
0.038 |
0.038 |
|
|
|
货期
3-5 working days