产品描述

This Human AIFM1 ELISA Kit was designed for the quantitative measurement of Human AIFM1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

别名

AIFM1 ELISA Kit; AIFM1_HUMAN ELISA Kit; Apoptosis inducing factor 1, mitochondrial ELISA Kit; Apoptosis inducing factor ELISA Kit; Apoptosis inducing factor, mitochondrion associated, 1 ELISA Kit; Apoptosis-inducing factor 1 ELISA Kit; CMTX4 ELISA Kit; COWCK ELISA Kit; COXPD6 ELISA Kit; Harlequin ELISA Kit; Hq ELISA Kit; mAIF ELISA Kit; MGC111425 ELISA Kit; MGC5706 ELISA Kit; mitochondrial ELISA Kit; Neuropathy, axonal motor-sensory, with deafness and mental retardation ELISA Kit; neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome) ELISA Kit; PDCD 8 ELISA Kit; PDCD8 ELISA Kit; Programmed cell death 8 (apoptosis inducing factor) ELISA Kit; Programmed cell death 8 ELISA Kit; Programmed cell death 8 isoform 1 ELISA Kit; Programmed cell death 8 isoform 2 ELISA Kit; Programmed cell death 8 isoform 3 ELISA Kit; Programmed cell death protein 8 ELISA Kit; Programmed cell death protein 8 mitochondrial ELISA Kit; Programmed cell death protein 8 mitochondrial precursor ELISA Kit; Programmed cell death protein 8 mitochondrial precursor ELISA Kit; Striatal apoptosis inducing factor ELISA Kit

缩写

AIFM1

Uniprot No

O95831

灵敏度

5.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human AIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		87-95
1:2		Average %		89
		Range %		85-94
1:4		Average %		88
		Range %		82-95
1:8		Average %		98
		Range %		92-103

回收率

The recovery of human AIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		91-98
EDTA plasma (n=4)		87		80-93

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1500 2.361 2.545 2.453 2.295 750 1.806 1.707 1.757 1.599 375 1.072 1.154 1.113 0.955 187.5 0.682 0.673 0.678 0.520 94 0.455 0.443 0.449 0.291 47 0.297 0.305 0.301 0.143 23.5 0.223 0.229 0.226 0.068 0 0.157 0.158 0.158

货期

3-5 working days