产品描述
The Rat brain natriuretic peptide (BNP) ELISA Kit is an accurate tool for the quantification of BNP in rat serum, plasma, and tissue homogenates. BNP is a hormone produced by the heart that plays a crucial role in regulating blood pressure and fluid balance,the condition of heart function can be judged by the examination of this item.This ELISA kit detects BNP with high sensitivity, with a detection range of 125 pg/mL to 8000 pg/mL and a sensitivity of 31.25 pg/mL.
The assay principle is a sandwich measurement, providing quantitative results that can be obtained in as little as 1-5 hours. The kit is optimized for use with a sample volume of 50-100ul, making it an efficient solution for researchers.
This ELISA kit is ideal for researchers in the area of cardiovascular disease research, as BNP has been shown to be a reliable biomarker of heart failure. Additionally, our kit has been widely cited in scientific literature, with over 13 publications referencing its use.
别名
NppbNatriuretic peptides B ELISA kit; Gamma-brain natriuretic peptide ELISA kit; Iso-ANP) [Cleaved into: Brain natriuretic peptide 45 ELISA kit; 5 kDa cardiac natriuretic peptide ELISA kit; BNP-34)] ELISA kit
缩写
BNP
Uniprot No
P13205
灵敏度
31.25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 80-92 | |
| 1:2 | Average % | 98 | |
| Range % | 91-105 | |
| 1:4 | Average % | 99 | |
| Range % | 92-110 | |
| 1:8 | Average % | 91 | |
| Range % | 86-98 | |
回收率
| The recovery of rat BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-99 | |
| EDTA plasma (n=4) | 95 | 90-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 8000 | 2.895 | 2.898 | 2.897 | 2.835 | | | 4000 | 2.294 | 2.326 | 2.310 | 2.248 | | | 2000 | 1.327 | 1.292 | 1.310 | 1.248 | | | 1000 | 0.707 | 0.689 | 0.698 | 0.636 | | | 500 | 0.345 | 0.317 | 0.331 | 0.269 | | | 250 | 0.196 | 0.184 | 0.190 | 0.128 | | | 125 | 0.117 | 0.114 | 0.116 | 0.054 | | | 0 | 0.064 | 0.060 | 0.062 | | |
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货期
3-5 working days
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