产品描述

    CASP3（Caspase-3），也称为细胞色素c依赖性半胱氨酸蛋白酶3，是一种重要的凋亡调节蛋白。CASP3可以通过一系列信号途径被激活，如线粒体通路、细胞色素C途径和死亡受体途径等，进而引起程序性细胞死亡（凋亡）。CASP3对于人体的维持和生存有着至关重要的作用，正常的组织和器官发育、代谢以及细胞周期等生理过程都需要CASP3的参与。

别名

Casp3 ELISA Kit; Cpp32Caspase-3 ELISA Kit; CASP-3 ELISA Kit; EC 3.4.22.56 ELISA Kit; Apopain ELISA Kit; Cysteine protease CPP32 ELISA Kit; CPP-32 ELISA Kit; LICE ELISA Kit; Protein Yama ELISA Kit; SREBP cleavage activity 1 ELISA Kit; SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12] ELISA Kit

缩写

CASP3

Uniprot No

P70677

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of mouse caspase 3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		95
		Range %		91-99
1:2		Average %		93
		Range %		87-97
1:4		Average %		103
		Range %		98-107
1:8		Average %		90
		Range %		84-95

回收率

The recovery of mouse caspase 3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		97		92-105
EDTA plasma (n=4)		92		88-97

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   20 2.637 2.552 2.595 2.437   10 2.175 2.106 2.141 1.983   5 1.464 1.524 1.494 1.336   2.5 0.832 0.829 0.831 0.673   1.25 0.543 0.524 0.534 0.376   0.625 0.366 0.339 0.353 0.195   0.312 0.269 0.285 0.277 0.119   0 0.159 0.157 0.158

货期

3-5 working days