产品描述
This Rat Col Ⅱ ELISA Kit was designed for the quantitative measurement of Rat Col Ⅱ protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .
别名
Collagen Type 2,Col 2,_x000D_
Collagen Type II,Col II
缩写
Col Ⅱ
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
87 |
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| Range % |
83-92 |
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| 1:200 |
Average % |
94 |
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| Range % |
90-99 |
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| 1:400 |
Average % |
92 |
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| Range % |
88-96 |
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| 1:800 |
Average % |
102 |
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| Range % |
98-105 |
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回收率
| The recovery of rat Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
93-96 |
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| EDTA plasma (n=4) |
98 |
89-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.584 |
2.599 |
2.592 |
2.438 |
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| 500 |
2.217 |
2.253 |
2.235 |
2.081 |
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| 250 |
1.765 |
1.792 |
1.779 |
1.625 |
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| 125 |
1.292 |
1.264 |
1.278 |
1.124 |
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| 62.5 |
0.789 |
0.773 |
0.781 |
0.627 |
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| 31.2 |
0.396 |
0.388 |
0.392 |
0.238 |
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| 15.6 |
0.257 |
0.269 |
0.263 |
0.109 |
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| 0 |
0.153 |
0.155 |
0.154 |
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货期
3-5 working days