产品描述
This human BMP15 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human BMP15 in multiple samples, including serum, plasma, or cell culture supernates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the BMP15 levels in the sample and is measured at 450 nm through a microplate reader.
BMP15 is expressed in oocytes and acts synergically with growth differentiation factor 9 (GDF9) towards the regulation of folliculogenesis. In addition to folliculogenesis, BMP15 is essential for female fertility. BMP15 and GDF9 polymorphism play vital roles in the pathogenesis of polycystic ovary syndrome (PCOS) and disturbed follicular steroidogenesis. BMP15 is closely related to animal reproduction and woman reproductive disease. As a multifunctional oocyte-specific secret factor, BMP15 controls female fertility and follicular development in both species-specific and dosage-sensitive manners. Mutations in BMP15 can cause both infertility and super ovulations in a dosage-sensitive manner.
别名
BMP 15 ELISA Kit; BMP-15 ELISA Kit; BMP15 ELISA Kit; BMP15_HUMAN ELISA Kit; Bone morphogenetic protein 15 ELISA Kit; GDF 9B ELISA Kit; GDF-9B ELISA Kit; GDF9B ELISA Kit; Growth/differentiation factor 9B ELISA Kit; ODG2 ELISA Kit; POF4 ELISA Kit
缩写
BMP15
Uniprot No
O95972
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human BMP-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 85-95 | |
| 1:2 | Average % | 98 | |
| Range % | 93-103 | |
| 1:4 | Average % | 93 | |
| Range % | 87-98 | |
| 1:8 | Average % | 96 | |
| Range % | 91-102 | |
回收率
| The recovery of human BMP-15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 102 | 96-107 | |
| EDTA plasma (n=4) | 92 | 86-97 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.765 | 2.637 | 2.701 | 2.519 | | | 500 | 2.245 | 2.178 | 2.212 | 2.030 | | | 250 | 1.376 | 1.483 | 1.430 | 1.248 | | | 125 | 0.756 | 0.784 | 0.770 | 0.588 | | | 62.5 | 0.467 | 0.481 | 0.474 | 0.292 | | | 31.2 | 0.335 | 0.321 | 0.328 | 0.146 | | | 15.6 | 0.235 | 0.245 | 0.240 | 0.058 | | | 0 | 0.183 | 0.181 | 0.182 | | |
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货期
3-5 working days
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