产品描述
Alpha-1-microglobulin/bikunin precursor (AMBP)是一种蛋白质,它由alpha-1-microglobulin (A1M)和bikunin(一种Kunitz型蛋白酶抑制剂)组成的前体蛋白。A1M是一种广泛分布的蛋白质,存在于许多组织和体液中,具有多种生物学功能,如抗氧化作用、免疫调节和铁离子绑定等。Bikunin则是一种强力的抗炎剂和抗凝剂。AMBP的生物学功能在很大程度上由A1M和bikunin的功能共同作用决定。AMBP在体内被认为参与多种生理和病理过程,包括肾脏疾病、糖尿病、心血管疾病等。因此,AMBP在临床上也作为一种生物标志物,用于评估这些疾病的风险和进展。
华美生物所提供的Rat alpha-1-microglobulin/bikunin precursor (AMBP) ELISA kit属于ELISA检测试剂盒,采用竞争法定量检测大鼠血清、血浆、组织匀浆样本中的AMBP,其灵敏度为0.649 ng/mL,检测范围为1.563 ng/mL-100 ng/mL。
别名
Ambp ELISA Kit; ItilProtein AMBP [Cleaved into: Alpha-1-microglobulin; Inter-alpha-trypsin inhibitor light chain ELISA Kit; ITI-LC ELISA Kit; Bikunin ELISA Kit; HI-30); Trypstatin] ELISA Kit
缩写
AMBP
Uniprot No
Q64240
灵敏度
0.649 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat AMBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
105 |
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| Range % |
99-109 |
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| 1:200 |
Average % |
96 |
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| Range % |
92-99 |
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| 1:400 |
Average % |
95 |
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| Range % |
90-98 |
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| 1:800 |
Average % |
89 |
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| Range % |
84-94 |
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回收率
| The recovery of rat AMBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
87-95 |
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| EDTA plasma (n=4) |
99 |
99-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
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| 100 |
0.116 |
0.124 |
0.120 |
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| 50 |
0.231 |
0.245 |
0.238 |
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| 25 |
0.478 |
0.498 |
0.488 |
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| 12.5 |
1.019 |
1.025 |
1.022 |
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| 6.25 |
1.426 |
1.367 |
1.397 |
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| 3.125 |
2.159 |
2.168 |
2.164 |
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| 1.563 |
2.466 |
2.544 |
2.505 |
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| 0 |
2.845 |
2.654 |
2.750 |
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货期
3-5 working days