产品描述
This Mouse BMP4 ELISA Kit was designed for the quantitative measurement of Mouse BMP4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 pg/mL-40 pg/mL and the sensitivity is 0.156 pg/mL.
别名
Bmp4 ELISA kit; Bmp-4 ELISA kit; Dvr-4Bone morphogenetic protein 4 ELISA kit; BMP-4 ELISA kit; Bone morphogenetic protein 2B ELISA kit; BMP-2B ELISA kit
缩写
BMP4
Uniprot No
P21275
灵敏度
0.156 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
80-95 |
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| 1:2 |
Average % |
96 |
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| Range % |
91-112 |
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| 1:4 |
Average % |
85 |
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| Range % |
80-110 |
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| 1:8 |
Average % |
90 |
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| Range % |
86-98 |
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回收率
| The recovery of mouse BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-98 |
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| EDTA plasma (n=4) |
92 |
90-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.657 |
2.677 |
2.667 |
2.509 |
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| 20 |
2.212 |
2.302 |
2.257 |
2.099 |
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| 10 |
1.583 |
1.683 |
1.633 |
1.475 |
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| 5 |
1.042 |
1.082 |
1.062 |
0.904 |
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| 2.5 |
0.597 |
0.622 |
0.610 |
0.452 |
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| 1.25 |
0.384 |
0.401 |
0.393 |
0.235 |
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| 0.625 |
0.288 |
0.302 |
0.295 |
0.137 |
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| 0 |
0.162 |
0.154 |
0.158 |
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货期
3-5 working days