产品描述

The human Corin ELISA Kit is used to quantitatively measure human Corin concentrations in serum, plasma, cell culture supernates, or tissue homogenates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of Corin in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.

Corin is a type II transmembrane serine protease that is predominantly located in the heart where it converts pro-atrial natriuretic peptide (ANP) to mature ANP, stimulating natriuresis, diuresis, and vasodilation thus regulating sodium homeostasis and blood pressure. Corin variants that impair Corin function have been found in patients with hypertension, heart disease, pre-eclampsia, and kidney disease. Defects in Corin may contribute to major diseases such as hypertension, heart failure, pre-eclampsia, and kidney disease. Corin is identified as a potential biomarker for gestational hypertensive disorders.

别名

activated protease fragment ELISA Kit; ATC 2 ELISA Kit; ATC2 ELISA Kit; Atrial natriuretic peptide-converting enzyme ELISA Kit; Atrial natriuteric peptide converting enzyme ELISA Kit; Corin ELISA Kit; Corin serine peptidase ELISA Kit; CORIN_HUMAN ELISA Kit; CRN ELISA Kit; Heart specific serine proteinase ELISA Kit; Heart specific serine proteinase ATC2 ELISA Kit; Heart-specific serine proteinase ATC2 ELISA Kit; MGC119742 ELISA Kit; Pro ANP convertase ELISA Kit; Pro ANP converting enzyme ELISA Kit; Pro-ANP-converting enzyme ELISA Kit; TMPRSS 10 ELISA Kit; TMPRSS10 ELISA Kit; Transmembrane protease serine 10 ELISA Kit

缩写

CORIN

Uniprot No

Q9Y5Q5

灵敏度

0.156 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human Corin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		87
		Range %		83-95
1:2		Average %		98
		Range %		94-102
1:4		Average %		102
		Range %		98-106
1:8		Average %		95
		Range %		90-100

回收率

The recovery of human Corin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		85-104
EDTA plasma (n=4)		104		88-108

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected     40 2.529 2.625 2.577 2.425     20 2.007 2.121 2.064 1.912     10 1.235 1.245 1.240 1.088     5 0.744 0.757 0.751 0.599     2.5 0.428 0.455 0.442 0.290     1.25 0.284 0.291 0.288 0.136     0.625 0.233 0.245 0.239 0.087     0 0.150 0.154 0.152

货期

3-5 working days