产品描述
This Pig CK-MB ELISA Kit was designed for the quantitative measurement of Pig CK-MB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 2.35 mU/mL-150 mU/mL and the sensitivity is 0.58 mU/mL.
缩写
CK-MB
灵敏度
0.58 mU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 93 | |
| Range % | 86-98 | |
| 1:2 | Average % | 95 | |
| Range % | 89-104 | |
| 1:4 | Average % | 95 | |
| Range % | 87-102 | |
| 1:8 | Average % | 95 | |
| Range % | 88-100 | |
回收率
| The recovery of pig CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 98 | 92-105 | |
| EDTA plasma (n=4) | 92 | 88-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| mU/ml | OD1 | OD2 | Average | Corrected | | | 150 | 2.802 | 2.791 | 2.797 | 2.607 | | | 75 | 2.360 | 2.314 | 2.337 | 2.147 | | | 37.5 | 1.705 | 1.716 | 1.711 | 1.521 | | | 18.75 | 1.127 | 1.113 | 1.120 | 0.930 | | | 9.37 | 0.615 | 0.602 | 0.609 | 0.419 | | | 4.68 | 0.424 | 0.438 | 0.431 | 0.241 | | | 2.35 | 0.325 | 0.319 | 0.322 | 0.132 | | | 0 | 0.191 | 0.189 | 0.190 | | |
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货期
3-5 working days