产品描述

The product CSB-EL004940HU is a sandwich ELISA kit developed to measure concentrations of human CD47 in serum, plasma, urine, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of CD47 in samples. The color intensity is measured at 450 nm via a microplate reader.

CD47 is a heavily glycosylated cell surface protein that regulates cellular phagocytosis by macrophages, transmigration of neutrophils, and activation of dendritic cells, T cells, and B cells through the interaction with its ligands including TSP-1, SIRPα, integrins, and SHPS-1. It plays an essential role in various cellular functions including proliferation, apoptosis, adhesion, migration, phagocytosis, and numerous immune responses. CD47 is overexpressed on the surface of many types of cancer cells. Ample studies have confirmed that high expression of CD47 aids many types of cancer to escape from the immune system. In many cancer types, CD47-SIRPα enables the escape of these cancer cells from macrophage-mediated phagocytosis.

别名

Antigen identified by monoclonal ELISA Kit 1D8 ELISA Kit; Antigenic surface determinant protein OA3 ELISA Kit; CD 47 ELISA Kit; CD47 ELISA Kit; CD47 antigen (Rh-related antigen; integrin-associated signal transducer) ELISA Kit; CD47 antigen ELISA Kit; CD47 glycoprotein ELISA Kit; CD47 molecule ELISA Kit; CD47_HUMAN ELISA Kit; IAP ELISA Kit; Integrin Associated Protein ELISA Kit; Integrin associated signal transducer ELISA Kit; Integrin-associated protein ELISA Kit; Leukocyte surface antigen CD47 ELISA Kit; MER 6 ELISA Kit; MER6 ELISA Kit; OA 3 ELISA Kit; OA3 ELISA Kit; OTTHUMP00000041152 ELISA Kit; OTTHUMP00000041153 ELISA Kit; Protein MER6 ELISA Kit; Rh related antigen ELISA Kit; Surface antigen identified by monoclonal ELISA Kit 1D8 ELISA Kit

缩写

CD47

Uniprot No

Q08722

灵敏度

0.78 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CD47 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		96
		Range %		90-101
1:2		Average %		106
		Range %		102-109
1:4		Average %		94
		Range %		90-98
1:8		Average %		88
		Range %		85-94

回收率

The recovery of human CD47 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		103		99-106
EDTA plasma (n=4)		93		91-95

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected     200 1.941 1.925 1.933 1.802     100 1.640 1.519 1.580 1.449     50 1.122 1.132 1.127 0.996     25 0.674 0.624 0.649 0.518     12.5 0.441 0.450 0.446 0.315     6.25 0.301 0.327 0.314 0.183     3.12 0.203 0.194 0.199 0.068     0 0.133 0.128 0.131

货期

3-5 working days